首頁  要聞  2013年工作回顧  媒體報道  要聞回顧  學習落實  圖片報道
  您的位置:首頁>要聞回顧
科學家揭示葉酸ECF轉運蛋白結構和轉運機制
發布時間:2013-04-17 來源:上海生命科學研究院 【字號:  

4月14日,《自然》雜志在線發表中科院上海生命科學研究院植物生理生態研究所的最新研究進展,報道了來源于乳酸桿菌的能量耦合因子型(Energy Coupling Factor,ECF)葉酸轉運蛋白面向內(inward-facing)的晶體結構(見示意圖a),揭示了ECF轉運蛋白跨膜轉運葉酸的分子機制。

葉酸參與細胞內眾多重要生化過程,包括DNA和氨基酸的合成。植物以及多數微生物能自身合成葉酸,而人和動物體不能合成葉酸。研究表明,人體葉酸攝入不足會導致胎兒神經系統發育的缺陷、巨幼細胞貧血等重大疾病。據統計我國居民中葉酸攝入不足的比例高達20%,每年有近10萬新生兒因葉酸缺乏導致神經系統發育缺陷。因此,通過育種途徑培育富含葉酸的作物品種對提高國民健康水平具有重要意義。雖然科學家們經過長期的研究闡明了葉酸的合成途徑,發現葉酸通過多種轉運蛋白在細胞或者細胞器之間穿梭,但關于其跨膜轉運的機制一直不清楚。

ECF轉運蛋白屬于新的ABC(ATP Binding Cassette)轉運蛋白,主要轉運多種B族維生素和微量元素。該復合體由細胞膜上底物結合蛋白S、和由膜結合蛋白T、胞內ATP結合蛋白A/A’組成的能量耦合模塊構成。ECF轉運蛋白分為耦合模塊專用型和共享型兩類:前者每一種S蛋白均有各自獨立的能量耦合模塊;后者有幾種不同的S蛋白,但共享相同的能量耦合模塊。與經典的ABC轉運蛋白中的內向轉運蛋白相比,其顯著特點在于不存在膜外底物結合蛋白BP(見示意圖b)以及不同S蛋白可以共享能量耦合模塊。

此次解析的葉酸ECF轉運蛋白結構是迄今第一個ECF型ABC轉運蛋白復合體的結構,也是葉酸跨膜轉運蛋白的首個結構。研究發現:四個組成該復合體的蛋白中,T蛋白的五個跨膜alpha螺旋與三個胞內負責能量耦合的alpha螺旋近似垂直,形成“L”型構象,兩個互相交叉呈“X”型的胞內alpha螺旋構成T蛋白的L型短臂;S蛋白則由六個跨膜alpha螺旋組成,斜插在膜內且躺在T蛋白“L”型短臂上方;A/A'蛋白并排排列在T蛋白“L”型短臂的下方,通過表面的凹槽與T蛋白中呈“X”型的兩個alpha螺旋兩端的精氨酸loop相互結合。

這一結構的解析揭示了ECF轉運蛋白與經典ABC轉運蛋白的顯著差異、能量耦合的結構基礎。結合轉運活性實驗以及與經典ABC轉運蛋白的比較分析,研究人員提出了葉酸通過ECF轉運蛋白跨膜轉運機制的模型(見示意圖c)。這是膜轉運蛋白研究領域的重大突破,一方面使人們對ABC轉運蛋白跨膜轉運的機理有了全新的理解,更為重要的是為人們理解維生素(特別是葉酸)如何跨細胞膜轉運進入細胞的過程邁出了一大步。同一期《自然》雜志報道了清華大學施一公研究組的另一ECF轉運蛋白的結構,得出了與此研究相似的結論。

該研究由中科院上海生科院植生生態所張鵬課題組完成,該課題組博士生許可、趙琴、助理研究員張敏華和生化細胞所丁建平課題組的助理研究員俞芳是文章的共同第一作者。該研究得到了科技部、上海市、中科院和依托單位的資助。上海光源BL17U線站在實驗數據收集中提供了支持與幫助。

http://www.178ing.com/ky/kyjz/201304/W020130415514910965642.jpg

葉酸ECF轉運蛋白結構和轉運機制

相關鏈接:

《自然》雜志在線發表論文原文

關閉頁面
? 1996 - 2014 中國科學院 版權所有 備案序號:京ICP備05002857號 聯系我們 地址:北京市三里河路52號 郵編:100864
百姓彩票www.www.bxcp3333.com www.321093.com | www.131594.com | www.261858.com | www.pr44.com | www.m25.online | www.286731.com | www.966792.com | 397246.com | www.149288.com | www.sc90.com | www.880320.com | www.5045.org | www.924944.com | www.299816.com | www.438268.com | www.602995.com | www.54rp.com | www.297433.com | www.653263.com | 357439.com | www.1674.org | www.458667.com | 446993.com | 948202.com | www.35uy.com | 930574.com | www.9219.pw | 506171.com | www.953001.com | www.22by.com | www.98in.cc | www.218976.com | www.389056.com | 016326.com | www.559747.com | 大赢家彩票平台www.106572.com | www.i67.org | www.77289.cc | www.525334.com | www.56481.cc | www.330838.com | www.7109.pw | www.4635.com | www.843012.com | www.61463.com | www.476687.com | www.8273.loan | 179562.com | 913956.com | www.zn89.com | www.798080.com | 743178.com | www.0851.date | www.03829.com | www.40104.com | www.3506.vip | www.976467.com | www.2355.pw | www.659071.com | www.1176.vip | www.162861.com | www.550785.com | www.448654.com | www.814980.com | www.750421.com | www.893095.com | www.111497.com | www.sl22.com | www.c93.vip | www.056063.com | 亚洲彩www.68568t.com | www.87199.cc | www.516121.com | www.75fn.com | www.357058.com | www.594433.com | www.949836.com | www.365278.com | www.569930.com | www.fk60.com | www.28868.com | 双彩网www.152012.com | www.l68.org | www.483985.com | 754954.com | www.01722.com | www.204446.com | www.546051.com | www.4772.biz | www.857968.com | www.163105.com | 036596.com | 254265.com | www.05410.com | www.68881.cc | www.103218.com | 0529.org | www.b80.cn | www.380246.com | www.9052.biz | 986777.com | www.252186.com | www.od55.com | www.qo43.com | www.829217.com | www.037371.com | www.515383.com | www.639564.com | www.cp3637.com | www.114332.com | www.xc61.com | www.03lo.com | www.7554.pw | www.69313.com | www.397746.com | 555106.com | 097635.com | www.33wx.cc | www.835680.com | 986467.com | 972840.com | 13e彩票www.973390.com | www.298215.com | www.ut57.com | www.036.biz | www.603592.com | www.22777.cc | www.274747.com | www.067761.com | 009079.com | 894834.com | 156664.com | www.680952.com | www.809619.com | www.928983.com | 591716.com | 423533.com | www.898544.com | www.801763.com | www.6lp.com | www.37075.com | www.387268.com | www.849681.com | www.4964.cm | 34088.cc | 乐点彩票www.775377.com | www.1407.me | www.3397.cn | www.980650.com | www.kt34.com | www.610722.com | www.09zg.com | 760822.com | 976372.com | www.q46.cn | www.077289.com | www.777856.com | www.16054.com | www.217038.com | 355962.com | 659470.com | www.038056.com | 732445.com | 459759.com | www.u49.com | www.654216.com | www.981497.com | www.6867.net | www.567439.com | www.84335.com | www.61296.com | www.037073.com | www.878657.cc | www.26kt.com | www.41029.com | www.97628.com | www.350448.com | www.90fb.com | www.621506.com | 62zc.com | www.260628.com | www.mx24.com | www.85as.com | www.129353.com | 074164.com | www.297464.com | www.092911.com | www.089196.com | www.mn37.com | www.74358.com | 200552.com | www.181405.com | 338436.com | 280178.com | www.cp4699.cc | www.19gc.com | 196502.com | www.wns981.com | www.701888.com | www.691672.com | www.aa89.cc | www.965007.com | 081097.com | www.522277.com | 974174.com | www.119467.com | www.803199.com | www.052.date | www.200065.cc | 599191.cc | www.382196.com | www.711803.com | www.39733.com | 908446.com | www.jj72.com | 351238.com | www.548.in | www.598859.com | www.676018.com | www.09039.com | www.67500.cc | www.814855.com | www.858345.com | www.83268.cc | www.863809.com | www.169854.com | www.967643.com | 344096.com | 883863.cc | www.508123.com | www.880085.com | www.295831.com | ji91.com | 537823.com | 219742.com | 611820.com | www.616828.com | www.914163.com | www.978185.com | www.384804.com | www.i86.xyz | www.100079.cc | www.105469.com | www.88483.com | www.515123.com | www.6934.shop | www.o26.net | www.483547.com | 845614.com | www.837300.com | 826604.com | www.286058.com | www.772808.com | www.082505.com | 100423.com | www.nv74.com | 537184.com | www.36478.com | www.240255.com | www.859287.com | www.022921.com | www.228368.com | 370384.com | 631077.com | 483543.com | 853694.com | www.ek20.com | 338059.com | 984176.com | www.948263.com | www.82mn.com | www.9133.pw | www.155658.com | www.295521.com | www.157342.com | www.305696.com | www.333382.com | www.307712.com | www.604218.com | 956864.com | www.118855.cc | 258309.com | www.173577.com | www.836397.com | www.8593.wang | www.262759.com | www.489655.com | 星际www.m.55xj.vip | 超级彩票www.901833.com | 053163.com | www.ev46.com | www.3615.love | www.8168.tv | 755848.com | www.948926.com | 网易彩票www.063wy.com | 506543.com | www.uf13.com | www.83ii.com | www.963654.com | www.049147.com | 汇彩网www.www.hcw266.com | 617665.com | www.fx42.com | www.18bu.com | 3227.cc | 507005.com | 290362.com | www.802049.com | www.447240.com | www.096623.com | www.048062.com | www.061098.com | www.219845.com | 308523.com | 385606.com | 639202.com | 526030.com | 563102.com | 254351.com | 678427.com | 099042.com | 365429.cc | www.677531.com | www.445419.com | www.509368.com | www.25kj.com | www.8565.biz | www.971486.com | 731909.com | www.cai9900.com | www.953665.com | www.89591.com | www.207297.com | www.553972.com | www.4165.cm | www.e20.com | www.970345.com | www.3794.com | www.075219.com | www.5310.cm | www.62bw.com | www.562.in | www.6369.vip | www.477420.com | 733178.com | 937227.com | www.527570.com | j65.me | 购彩网www.73gcw.com | www.zu4.com | www.k78.net | www.192.cn | www.120019.com | www.479678.com | www.j45.xyz | 中国福利彩票www.33588e.com | 993645.com | 761154.com | 737559.com | 127894.com | 914773.com | www.925929.com | www.653334.com | www.145611.com | www.542014.com | www.711837.com | www.789459.com | 126405.com | 666527.com | 1587.me | www.di88.com | www.kl41.com | www.57763.cc | www.67089.cc | www.132429.com | www.495007.com | 155369.com | www.42659.com | 732163.com | www.i19.net | www.127583.com | www.58jp.com | www.075358.com | www.549623.com | 847548.com | www.80bo.com | 870046.com | 343896.com | www.cai7600.com | www.39572.cc | 8号彩票www.60108g.com | www.362821.com | www.731374.com | www.wv34.com | www.65vq.com | www.90528.com | www.53948.com | www.73949.com | 25699.com | 29dr.com | www.968361.com | www.8478.com | www.062771.com | www.70766.com | www.900038.cc | www.848355.com | www.934302.com | 781495.com | www.947066.com | www.676518.com | www.253836.com | 582834.com | www.71883.com | 399777.com | www.758991.com | www.46xt.com | www.26bz.com | www.640222.com | www.64985.com | www.758844.com | www.691168.com | www.963617.com | www.893773.com | 127516.com | www.137526.com | www.856151.com | www.35.com | www.280976.com | 899074.com | 005943.com | www.409494.com | www.4251.vip | 905282.com | 677.com | 双彩网www.363991.com | www.61224.com | www.253919.com | www.536.date | 974125.com | www.694552.com | www.676516.com | www.512068.com | www.957290.com | www.004005.com | www.249837.com | www.017194.com | www.071137.com | www.256955.com | www.566663.com | www.233676.com | www.189195.com | www.242516.com | www.189576.com | www.160621.com | www.611098.com | 962849.com | 602451.com | www.hq56.com | www.999416.com | www.077025.com | 363350.com | www.246640.com | www.25kj.com | www.456683.com | www.0477.space | www.035083.com | www.023951.com | www.756532.com | 319559.com | www.949623.com | 139247.com | 彩多多www.78949e.com | www.667485.com | www.79929.com | www.859640.com | 948960.com | www.962092.com | www.962014.com | www.911879.com | 410243.com | 446410.com | www.006191.com | www.11400.cc | www.rd70.com | www.741010.com | www.35337.cc | www.18ly.com | www.882588.com | www.001984.com | 448635.com | www.444276.com | 彩多多www.78949l.com | www.5741.cc | www.1035.live | www.3565.biz | www.52262.com | www.109411.com | www.122206.com | www.024148.com | www.33007.com | www.998297.com | www.432507.com | 528908.com | www.309099.com | www.12yj.com | www.739329.com | www.01261.com | www.970425.com | www.033003.com | 313523.com | www.425205.com | www.4272.top | www.869831.com | www.397283.com | www.017169.com | 240976.com | www.396058.com | www.3726.vip | www.865963.com | www.171552.com | www.8078.tv | 176506.com | www.405805.com | 网易彩票www.091wy.com | 050664.com | www.pn22.com | PK彩票www.840774.com | 679752.com | www.876393.com | www.556201.com | 307826.com | www.205909.com | www.cp6679.com | www.553608.com | www.p10.org | www.ys88.com | www.81ym.com | www.3050.vip | www.562518.com | www.434585.com | www.349355.com | www.280282.com | www.218234.com | www.179795.com | www.404141.com | www.5590.vip | www.610252.com | www.723625.com | www.417614.com | www.871498.com | 825638.com | www.ed92.com | www.io60.com | 878737.com | 639509.com | 748995.com | 174035.com | www.fk81.com | www.cx00.com | www.78.cx | 网易彩票www.332910.com | www.6111.com | www.4080.pw | www.z57.com | 211743.com | www.974584.com | www.097908.com | www.42pa.com | www.693001.com | www.639453.com | www.031658.com | 324771.com | www.166947.com | www.15ii.com | www.933932.com | www.336739.com | www.fp12.com | www.770549.com | www.37673.cc | www.766444.com | www.619125.com | 凤凰彩票www.88266k.com | www.760148.com | 502071.com | 503660.com | 652326.com | www.wd7.com | 998155.com | www.we45.com | www.557.in | www.4680.in | www.171999.com | www.205928.com | www.549550.com | www.986303.com | www.854880.com | 509851.com | 230252.com | www.975958.com | www.80wq.com | www.vx53.com | www.nl04.com | www.rk3.com | 016628.com | 37566.com | 127864.com | 115071.com | www.992827.com | www.839296.com | 145012.com | 418611.com | www.cl83.com | www.pl87.com | www.ch30.com | QQ彩票www.89894m.com | www.9280.vip | www.53667.cc | www.030158.com | www.028523.com | www.166567.com | www.351313.cc | www.335992.com | www.163832.com | www.122773.com | www.281591.com | www.56379.cc | www.335630.com | www.929590.com | www.731981.com | 443667.com | www.813727.com | www.813325.com | 982142.com | www.374598.com | www.029560.com | 948304.com | www.954708.com | www.526275.com | www.21551.cc | www.949216.com | 830998.com--www.550989.com | www.555043.com | www.61md.com | www.870344.com | www.265973.com | 彩82www.c5128.com | www.486999.com | www.rr56.com | www.31848.com | www.80pz.cc | www.316166.com | www.910482.com | www.79050.com | 606651.com | www.572216.com | www.1988.name | www.970412.com | www.764183.com | www.617287.com | www.32.biz | 207722.com | www.436429.com | www.2513.biz | www.782722.com | www.149633.com | www.449888.com | www.706381.com | 570316.com | www.238898.com | www.862686.com | www.866135.com | www.835585.com | www.835871.com | www.877115.com | 398719.com | www.861696.com | www.947930.com | www.667552.com | www.901314.com | www.ku2.cc | yh61.cc | 565220.com | 097135.com | www.978292.com | www.830502.com | 647611.com--www.4887.con | 577552.com | 488489.com | qw07.com | www.j56.me | www.789058.com | www.2881.xyz | www.3lc.cc | www.su63.com | www.e01.xyz | www.02rx.com | www.xb91.com | 455163.com | 977722.com | 688563.com | 127105.com | 924984.com | www.32646.com | www.5998.biz | www.03qi.com | 来运彩票www.6666y.cc | 659709.com | 824099.com | www.709482.com | www.ll59.com | www.j11.org | www.cai5377.com | www.cai9677.com | www.uv69.com | www.2393.biz | www.1726.vip | www.838.in | www.25579.cc | www.28ed.com | www.85wx.com | www.p01.net | 幸运彩票www.672166.com | 089950.com | www.3600.in | www.812531.com | www.07052.com | www.896236.com | www.69yz.com | www.hz07.com | 中国福利彩票www.33588s.com | 900907.com | www.789823.com | www.901595.com | www.693151.com | www.546672.com | www.762792.com | www.6514.biz | www.901440.com | www.26256.cc | 942513.com | www.192632.com | www.380406.com | www.337957.com | www.cp0633.com | 44579.com | 947470.com | 964003.com | 467097.com | www.914929.com | 467557.com | www.459429.com | www.2pc.com | www.673972.com | 237742.com | tk48.com | 58049.com | www.0546.org | www.js44.com | 672176.com | www.30jg.com | 429297.com | www.349356.com | www.ld0.com | www.566301.com | www.50vo.com | www.662672.com | www.52216.cc | 手机购彩www.525988.com | 506206.com | 085583.com | www.263322.com | 1502.me | www.330690.com | 206151.com | www.535865.com | www.2983.pw | www.874019.com | www.198966.com | 亚洲彩票联盟www.786778.com | www.501271.com | www.27wb.com | 443529.com | www.671869.com | www.75mz.com | www.hw08.com | 易彩网www.36166f.com | 886484.com | 435311.com | 000142.com | 301295.com | 910860.com | 417036.com | 410161.com | 792301.com | www.20.la | 741374.com | 647920.com | 957024.com | www.953162.com | www.676746.com | www.411665.com | 060799.com | www.19.org | www.72pn.com | www.im86.com | 大赢家彩票平台www.976981.com | 302416.com | www.ew17.com | www.cp3372.com | nx39.com | www.993332.com | www.931629.com | www.611782.com | www.329557.com | www.224101.com | www.728782.com | www.669792.com | www.975601.com | www.931521.com | www.846128.com | 777966.com | www.948831.com | www.685207.com | www.814412.com | www.690038.com | www.576473.com | www.588861.com | www.607318.com | 669054.com | 960747.com | www.879795.com | 09079.com | 764277.com | www.945611.com | 891842.com | 乐点彩票www.239828.com | 815881.com | 170596.com | 969284.com | www.920250.com | www.629122.com | www.930631.com | www.737951.com | www.395585.com | www.524380.com | www.052203.com | www.100227.com | www.593575.com | www.308558.com | www.733438.com | www.882711.com | 大赢家彩票www.346511.com | cai6464.com | www.16071.com | 300彩票www.c300.vip | www.373545.com | www.7827.cn | www.884974.com | www.81378.cc | 239756.com | www.160844.com | 657250.com | www.639184.com | www.8499.vip | www.995990.com | www.304969.com | 680627.com | www.513882.com | www.5374.org | www.483777.com | www.782199.com | www.888766.com | www.681295.com | www.848557.com | www.977113.com | 182888.com | 024745.com | www.854722.com | 594973.com | www.974930.com | www.700632.com | www.613569.com | www.41uc.com | 500彩票www.50064l.com | 819249.com | www.988719.com | www.800954.com | www.0672.xyz | www.0062.loan | www.32083.com | www.54fa.com | 开心彩票www.367137.com | 502823.com | 173580.com | www.761733.com | www.450598.com | www.278863.com | www.052996.com | www.743101.com | www.497649.com | www.189606.com | www.802183.com | www.702565.com | www.552553.com | www.817212.com | 169010.com | www.936701.com | www.709983.com | www.594460.com | www.190