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冷凍的未成年恒河猴生殖組織可繁育后代

文章來源:科技日報 劉海英   發布時間:2019-03-22  【字號:     】  

  美國研究人員在最新一期《科學》雜志上發表研究報告稱,他們成功利用冷凍的未成年恒河猴睪丸組織培育出成熟精子,使雌猴受孕并生育出健康的猴寶寶。這一研究結果表明,低溫保存未成熟睪丸組織或可幫助解決兒童癌癥幸存者的未來不育問題。

  對于男性癌癥患者來說,化療和放療等治療手段會極大影響其產生活精子的能力,有時會導致永久性不育。為此,一些成年男性在進行此類治療前,會將其精子冷凍保存以備將來之需。但這種手段卻難以解決男性兒童癌癥患者的未來生育問題。有研究表明,存活下來的男性癌癥患兒中,有30%的幸存者會在成年后不育。對這些年輕患者來說,冷凍保存含有精原干細胞的未成熟睪丸組織,在患者長大成人后將其“嫁接”回來,或是一個選擇。這種方法在其它動物(如小鼠和豬)中被證明是成功的,對于人類來講在理論上也是可行的。但科學家此前在靈長類動物中的嘗試基本上都沒有成功。

  為了評估使用該方法保存人類生育能力的可行性,匹茲堡大學醫學院凱爾·奧維格博士領導的研究小組,在美國國家兒童健康與人類發展研究所資助下開展了一項以恒河猴為對象的專項研究。研究人員從5只尚不能產生精子的未成年恒河猴體內取出睪丸組織并將其冷凍保存,待這些恒河猴接近青春期時,再將組織樣本解凍并重新移植到原主體內。幾個月后,研究人員取出植入組織,發現它們都產生了精子。隨后,研究人員用從這些組織中分離出的精子對138個卵子受精,結果41%的受精卵發育成了早期胚胎。研究人員將11個胚胎分別植入雌性恒河猴子宮中,結果有一只恒河猴成功受孕并產下一個健康的猴寶寶。

  研究人員指出,雖然他們的研究規模不大,但對驗證使用未成熟睪丸組織來保存人類生育能力的可行性很有幫助。而在該技術適用于人類之前,還需要進行更多的研究。




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